Their results reaffirmed that 3D culture decreased chemosensitivity to tamoxifen, fulvestrant, and trastuzumab; further, they found that 3D culture promotes switching from the phosphatidylinositide-3-kinase/protein kinase B (PI3K/AKT) to the mitogenic activation of protein kinase (MAPK) signaling pathway, illustrating the importance of 3D culture in studies of cancer biology. The gene discussed is AKT1; the disease is cancer.