In addition, western blot analysis and caspase-3 activity assay further revealed that acetyl-bufalin dose-dependently increased the expressions of the proapoptotic proteins, BAX and caspase-3 activity, and decreased the level of the anti-apoptotic protein, BCL2, in human NSCLC cells (Fig. 2c, d and Supplementary Fig. S2c). This evidence concerns the gene BAX and non-small cell lung carcinoma.