In addition, the positive rates of cytoplasmic HMGB1 in normal colorectal tissue, colorectal adenoma and CRC showed a gradually increasing trend, with significantly higher expression of positive cytoplasmic HMGB1 in high-grade versus low-grade CRC tissue, suggesting that cytoplasmic HMGB1 expression is closely related to CRC tumor grade. Here, HMGB1 is linked to neoplasm.