Univariate analysis results showed different factors were all correlated with a shorter OS: AML transformation (mOS: 447 days and 802 days, p = 0.028), chromosome 7 abnormality (-7, del(7q)) (mOS: 400 days and 830 days, p < 0.001), chromosome 20 abnormality (del(20q)) (mOS: 412 days and 802 days, p = 0.047), EZH2 mutation (mOS: 256 days and 791 days, p = 0.005), TP53 mutation (mOS: 497 days and 7 97 days, p = 0.023), CBL mutation (mOS: 158 days and 791 days, p = 0.038), RUNX1 VAF > 40% (mOS: 432 days and 909 days, p = 0.034), U2AF1 VAF > 40% (one-year OS rate: 46.1% and 80.5%, p = 0.027). The gene discussed is U2AF1; the disease is acute myeloid leukemia.