In the p53-wild-type HCT-116 CRC cell line, CX-5461 was shown to inhibit RNA pol I transcriptional activity by disrupting the binding of SL1/TIF-IB transcription factors to the rDNA promoter on the PIC complex, without affecting general transcription and global protein synthesis, thereby promoting nucleolar stress, stabilization of p53, and cell death [179,180]. Here, TP53 is linked to colorectal carcinoma.