Thus, when 2D-micropipette is used to look at antigen-reactivity of CD4+ T cells in autoimmune diseases such as the Experimental Autoimmune Encephalomyelitis (EAE) mouse model of Multiple Sclerosis (MS), the frequency of autoreactive CD4+ T cells is much higher than previously appreciated using tetramer staining. The gene discussed is CD4; the disease is multiple sclerosis.