Thus, Li et al. [74] compared different second and third generation CAR constructs in NK cells derived from iPSC cells, and demonstrated that CARs with NKG2D as transmembrane domain and 2B4 as co-stimulatory domain were crucial for activating endogenous signaling and conferring strong NK cell anti-tumor cytotoxicity, compared to CAR-NK with T cell domains. The gene discussed is KLRK1; the disease is neoplasm.