Results revealed shVE‐cadherin or shNotch4 alone could partially reverse the formation of HCC VM that had been increased by shAR shVE‐cadherin and shNotch4 together completely reversed the formation of HCC VM that had been increased by shAR. These results confirmed that AR suppressed the formation of HCC VM via inhibiting the expression of Notch4 and VE‐cadherin. The gene discussed is NOTCH4; the disease is hepatocellular carcinoma.