STK35 and colorectal carcinoma: We examined both the mRNA and protein levels of STK35 in a normal human intestinal crypt cell line and various CRC cell lines (Supplementary Figure S2A), following which, we selected SW620 (Supplementary Figure S2B) and HCT116 (Supplementary Figure S2C) cells with the highest expression of STK35 for use in gene knockdown experiments.