To explore the role of PHF20 in GBM tumorigenesis, we firstly determined the PHF20 expression in GBM, the protein level of PHF20 in ten primary GBM cell lines was tested by western blotting: BT115, BT135, BT136, BT139, BT141, BT145, BT147, BT149, BT150, and BT156 (Supplementary Figure 1A). This evidence concerns the gene PHF20 and glioblastoma.