Knockdown of NSD2 and NSD3 gene via siRNA transfection results in decrease of H3K36me2 and 3, and H3K36me2 levels, respectively, leading to significant reduction of HNSCC cells (UD-SCC2, UM-SCC35, HN-SCC151, PE/CA-PJ15; UD-SCC2, YD-10B, HN13) viability in vitro and indicating that they play important roles in H3K36 methylation in HNSCC. This evidence concerns the gene NSD3 and head and neck squamous cell carcinoma.