We first downloaded the mRNA expression data of normal and bladder cancer tissues from TCGA database and analyzed the mRNA expression of a total of 97 RNA splicing proteins (including SRSF family, RBM family, HNRNP family, SF3B1, PRMT5, PUF60, U2AF1, and ZRSR2) between normal and carcinoma tissues. Here, U2AF1 is linked to carcinoma.