Among the up-regulated genes in the PUF60high group, 12 out 40 were those promoting the proliferation and progression of bladder cancer or serving as independent unfavorable biomarkers for patients, including MCM2 (45), CKS1B (46), CDCA8 (47), TK1 (48), RAD21 (49), AURKA (42, 44), CDCA5 (50), TRIP13 (51), TACC3 (52), IQGAP3 (53–55), CDC20 (56, 57), RECQL4 (58). The gene discussed is RECQL4; the disease is urinary bladder cancer.