We first downloaded the mRNA expression data of normal and bladder cancer tissues from TCGA database and analyzed the mRNA expression of a total of 97 RNA splicing proteins (including SRSF family, RBM family, HNRNP family, SF3B1, PRMT5, PUF60, U2AF1, and ZRSR2) between normal and carcinoma tissues. This evidence concerns the gene PUF60 and urinary bladder carcinoma.