In line with this, TCTP overexpression on colon cancer cell lines resulted in the release of HMGB1 from the nucleus to the cytoplasm and into the extracellular space led to activation of NF-κB through the RAGE/TLR4/HMGB1 mediation, which simplified CRC cell invasion. The gene discussed is HMGB1; the disease is malignant colon neoplasm.