To investigate immunological effects related with the protection against primary infection, the numbers of CMFO-specific IFN-γ+ (or IL-2+) T cells, IL-2+ TCM (CD62LhiCD44hi) cells, and IFN-γ+ TEM (CD62LloCD44hi) cells in splenocytes from different vaccinated mice were determined by fluorescence activated cell sorting (FACS) before (Figures 7A, B) and after infection (Figure 8). Here, IL2 is linked to infection.