PD-1 blocking increased not only CD8 T cells but Tregs as well, while relative Foxp3 gene expression decreased when the tumor-bearing mice were treated with anti-TIGIT:mIgG2a, suggesting functional attenuation of intracellular Tregs or/and a proportional decrease of Treg population by other cell-types, such as myeloid cells as shown in Figure 6F and as suggested by others (7). The gene discussed is TIGIT; the disease is neoplasm.