Macrophages were also genetically edited for cell-based cancer immunotherapy through the elimination of CD47:SIRP-α interactions by knocking out SIRP-α using the CRISPR-Cas9E20 system and by introducing an E20 tag at the protein N terminus, thus facilitating self-assembly with arginine-coated gold nanoparticles (ArgNPs). The gene discussed is SIRPA; the disease is cancer.