We also made a set of genes that are expressed at the motor endplate and are associated with myasthenia gravis and postsynaptic congenital myasthenic syndromes such as Chrne, Musk, and Dok7. GSEA analysis showed that these genes were enriched at the motor endplate of TA muscle but without statistical significance, which was likely due to the limited number of genes (p = 0.070, Supplementary Figure S1 and Supplementary Table S5). The gene discussed is CHRNE; the disease is myasthenia gravis.