CD274 and cancer: 35 KEGG items were identified, including T cell mediated immune related pathway, natural killer cell mediated cytotoxicity, T helper cells differentiation, PD-L1 expression and programmed death‐1 receptor (PD-1) checkpoint pathway in cancer (Table 3), and CTLA4 was positively correlated with T cell receptor signaling pathway (cor = 0.80), Natural killer cell mediated cytotoxicity (cor = 0.75), Th1 and Th2 cell differentiation (cor = 0.75) and Th17 cell differentiation (cor = 0.78) (Fig. 2a).