Furthermore, RT-qPCR and Western blot analysis were conducted to measure the expression patterns of ERRFI1 in glioma tissues, which revealed that the expression of ERRFI1 was lower in tissue samples from patients with anaplastic astrocytoma, anaplastic oligodendrogliomas, oligodendroglioma, astrocytoma, and glioblastoma than that in the non-neoplastic brain tissue samples (p < 0.0001) (Fig. 4e, f). This evidence concerns the gene ERRFI1 and astrocytoma (excluding glioblastoma).