It was therefore surprising that neither in MM cell lines engineered for doxycycline-inducible HUWE1 knockdown nor in the MYC-overexpressing subline U266-MYC, moderate to strong HUWE1 depletion had any discernible effects on the expression levels of MYC target genes, nor on MIZ protein, an inhibitor of MYC activity. This evidence concerns the gene HUWE1 and Miyoshi myopathy.