Finally, we analysed a number of regulatory factors known to interact with HUWE1 and to be associated with DDR (e.g. γH2AX, BRCA1, Mcl1, p53, DNA-Polymerase β8–10,17) by Western blotting of MM cells with and without HUWE1 depletion (Fig. 4d). Here, HUWE1 is linked to Miyoshi myopathy.