Our data show that inhibition of RhoA-mediated signaling, latrunculin B-induced depolymerization of actin, and myosin II ATPase inhibition by blebbistatin reduced cytokine-induced YAP/TAZ activation in differentiated adipocytes, suggesting that increased cytoskeletal tension in obese adipocytes contributes to YAP/TAZ activation during obesity. Here, RHOA is linked to obesity disorder.