As indicated in Fig. 4A, expression of raptor (a component of mTORC1) and the mTORC1 downstream targets phospho-S6 (Ser240/244) and phospho-4EBP-1 (Thr37/46) were significantly demonstrated, accompanying with phosphorylation of mTOR at Ser2448 in clinical HCC samples (n = 20). The gene discussed is EIF4EBP1; the disease is hepatocellular carcinoma.