In order to study the characteristics of CTNNB1 mutations in CRC and their association with altered E-cadherin expression, we evaluated 869 CRCs for CTNNB1 mutations and analysed the expression of E-cadherin and β-catenin in three cell lines with CTNNB1 wildtype (DLD1), heterozygous (HCT 116), and homozygous mutations (LS 180). This evidence concerns the gene CDH1 and colorectal carcinoma.