Some KEGG signaling pathways (IL17, TNF, Chemokines, ErbB) as well as cancer-associated pathways (Proteoglycans in cancer, Pathways in cancer, Bladder cancer, Breast cancer, Basal cell carcinoma) were enriched after 4 h EGF treatment (Table S5, Additional file 4). The gene discussed is EGFR; the disease is urinary bladder carcinoma.