In this study, gas chromatography was used to determine the metabolome expression in cell lines derived from acute myelogenous leukemia (AML), chronic myelogenous leukemia (CML), and cord blood (V-derived CD34 positive hematopoietic stem cells (HSCs) and to select a common metabolome between AML and CML for the identification of novel putative diagnostic biomarkers for leukemia. This evidence concerns the gene CD34 and acute myeloid leukemia.