CD274 and non-small cell lung carcinoma: Recently in vitro study showed that treatment with pemetrexed enhanced PD-L1 expression, both in its membrane-bound form and its soluble form, in non-squamous NSCLC cell lines through activation of mTOR and STAT3 signaling pathways, whereas other chemotherapeutic agents (e.g., gemcitabine, paclitaxel, vinorelbine, cisplatin) did not influence PD-L1 levels [112].