With NFAT5 playing a role also in fibroblast function, DMD being characterized by chronic inflammation and DMD patients suffering permanent production of fibrotic tissue in skeletal muscles, we studied the localization and expression of NFAT5 in healthy and DMD skeletal fibroblasts of one healthy individual and one affected by DMD, after exposure to hyperosmolar or pro-inflammatory stress. Here, NFAT5 is linked to Duchenne muscular dystrophy.