NF1 and glioblastoma: By using stable transfections with lentiviruses carrying shRNAs that specifically knockdown NLS- or ΔNLS-NF1 transcripts in the human glioblastoma cell line SF268, we were able to produce glioblastoma cellular backgrounds that conditionally express either NLS- or ΔNLS-NF1 transcripts (Supplementary Figure S1).