To further elucidate the mechanism underlying the anti-obesity effects of BS21, we measured marker proteins of BAT (PR domain containing 16 (PRDM16), peroxisome proliferator-activated receptor gamma co-activator 1-alpha (PGC1α), and UCP1) involved in adaptive thermogenesis and the transition of WAT to brown-like adipose tissue [25]. Here, PRDM16 is linked to Obesity.