However, levels of the active/spliced form of X-box-binding protein 1 (XBP-1s) protein in nuclear extracts were not significantly modified by any treatment, and mRNA levels of the XBP-1s target gene ER degradation-enhancing α-mannosidase-like 1 (Edem1) were not altered by HF-HFr diet and showed reduced expression in the CAF group (Figure 7B,C). The gene discussed is EDEM1; the disease is hydrops fetalis.