Of the 43 genes, 24 are known to be enriched in breast cancer tissues, of which 9 are also enriched in normal breast epithelium (Table 3), and 15 genes, namely ELOVL6, LHX2, CLIC6, CAMK2N1, FAT1, TFAP2B, HOXA11, TOLLIP, SEPT9, JAKMIP1, CAND2, PODNL1, C12orf41 (KANSL2), GLS2, and NCOR1, harbored significant methylation changes consistent with their differential expression in breast cancer. This evidence concerns the gene GLS2 and breast cancer.