Accordingly to this hepatoprotective role of TGR5 when BA are increased, it was expected that the ablation of TGR5 would not improve cholestasis, which is consistent with our data concerning cholestasis, as shown in Table 1 (increased ALT, ALP, and total bilirubin) and Supplementary Figure S1 (increased plasma BA). Here, GPBAR1 is linked to cholestasis.