Using Kaplan- Meier method and log-rank test, there were significant associations between shorter overall survival of HCC and larger tumor size (p =0.0001), tumor grade (p =0.0001), high stage (p =0.0001, tumor multiplicity (p =0.0001), presence of cirrhosis (p =0.020), tumor necrosis (p =0.027), high p53 expression (p=0.0001), positive β-catenin expression (p=0.009), positive SOX2 expression (p=0.0001) and positive SOX9 expression (p=0.0001). This evidence concerns the gene SOX9 and hepatocellular carcinoma.