In this study, we discovered the role of LINC00265, SAT1 and VAV3 in osteosarcoma through bioinformatics and analysed the mechanism by which LINC00265 promotes the migration, invasion and tube formation of osteosarcoma cells via miR-382-5p targeting of SAT1 and VAV3. This evidence concerns the gene LINC00265 and osteosarcoma.