Immunohistochemical analysis of the tumors in control and RAGE shRNA treated cells injected in nude mice showed significantly higher angiogenesis (CD34), leukocyte (CD45) and macrophage (F4/80) markers level in control shRNA compared to RAGE shRNA treated MDA MB-231 and MCF-7 (Fig. 6e, f) injected nude mice and treated further with LPA confirming role of RAGE in LPA mediated mammary tumorigenesis and immune cells infiltration in tumor microenvironment. This evidence concerns the gene CD34 and neoplasm.