Immunohistochemical analysis of the tumors in control and RAGE shRNA treated cells injected in nude mice showed significantly higher angiogenesis (CD34), leukocyte (CD45) and macrophage (F4/80) markers level in control shRNA compared to RAGE shRNA treated MDA MB-231 and MCF-7 (Fig. 6e, f) injected nude mice and treated further with LPA confirming role of RAGE in LPA mediated mammary tumorigenesis and immune cells infiltration in tumor microenvironment. Here, AGER is linked to neoplasm.