Expression of EVs cancer markers, such as MUC1-CD277, a transmembrane protein involved in metastasis, A33 a glycoprotein found on most primary and metastatic colorectal cancers, and epithelial cell adhesion molecule (EPCAM), a transmembrane glycoprotein involved in tumorigenesis, were explored. The gene discussed is GPA33; the disease is metastatic colorectal cancer.