In an effort to understand how inactivating mutations of CASP8 that result in loss of protein function affect necroptosis sensitivity in HNSCC, we stably knocked down CASP8 using a short hairpin RNA (shRNA) in 2 CASP8 WT HNSCC cell lines, namely the human-derived UMSCC 17A cells and mouse-derived syngeneic oral cancer MOC1 HNSCCs (33). Here, CASP8 is linked to head and neck squamous cell carcinoma.