We have shown that expression of certain genes specific to AIS in CD8(+) T-cells provided 66%, 87%, and 100% clinical sensitivity for 2.4 h, 5 h, and 24 h following stroke onset33, suggesting that mRNA expression differences indicative of AIS have improved clinical sensitivity with time as more leukocytes respond to an inflammatory insult. The gene discussed is CD8A; the disease is androgen insensitivity syndrome.