Quantitative cell analysis using CellProfiler on selected cases showed the following percentages of CD68 and CD163 double-positive and single-positive macrophages per all nucleated cells: PMF: 60%, 62%, 53%; PV: 42%, 80%, 40%; ET: 56%, 70%, 52%; CML: 18%, 26%, 16% (percentage of CD68 single-positive, CD163 single-positive and double-positive cells respectively). Here, CD163 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.