HSPA5 and cancer: (D) Quantified ratio of spliced and unspliced measured on agarose gel after digestion by Pst-I (E) mRNA expression of HSPA5 in stellate cells (LX2) co-cultured with cancer cells (HepG2 or Huh7) and treated with 4μ8C or control. (F) protein expression of p-IRE1α and vinculin in stellate cells (LX2) co-cultured with cancer cells (HepG2 or Huh7) in transwell assays and treated with 4μ8C or control.