Our in vivo study also revealed the damage of mitochondrial outer membrane and lack of cytochrome c after 30 min of ischemia/reperfusion (cytochrome c test), which increased drastically (two and four times) after 40 min and 60 min of ischemia/reperfusion respiring on succinate and supported our results in vitro [14]. The gene discussed is CYCS; the disease is ischemia.