In order to accurately clarify the dynamic distribution of CTHRC1 in various organs upon ALV-J infection and the expression of CTHRC1 with the extension of infection time, SPF chick embryos were inoculated with ALV-J in the allantoic cavity on the 6th day of incubation, and then chicks were euthanized at 15, 22, and 35 days post-infection. Here, CTHRC1 is linked to infection.