As shown in Figure 8A, Supplementary Figures 3A, 4D the transfection efficiency was examined by RT-PCR and western blot, CCK-8 assays indicated that knocking down PPAT, YWHAB expression suppressed the proliferative ability of HCC cells, while the effect of NOL10 on HCC growth is insignificant (Figure 8B and Supplementary Figure 3B). The gene discussed is NOL10; the disease is hepatocellular carcinoma.