In order to simultaneously analyze the expression of the three TAM-R, we generated dendritic cells (DC) in vitro from monocytes using granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, various in vitro differentiated macrophages, i.e., M1, M2a, and M2c as described by Martinez et al. (23), and tumor-associated-like macrophages (TCM), generated with conditioned supernatant from triple-negative breast cancer cell line MDA-MB-231 (24). Here, CSF2 is linked to triple-negative breast carcinoma.