TNF and Sepsis: In mice subjected to CLP and fed a HFD, but not treated with Candida, sepsis were more severe than regular diet mice as determined by survival, organ injury (serum creatinine and alanine transaminase), serum cytokines (TNF-α and IL-6), gut leakage by FITC–dextran and endotoxemia, but not serum BG (Figures 3A–I).