TNF and obesity due to melanocortin 4 receptor deficiency: In mice fed a HFD but not subjected to CLP, Candida did not worsen obesity-induced liver injury, gut leakage, and serum cytokines, but it did activate local inflammation (colon TNF-α and IL-6; Figures 6C–H) without diarrhea when compared with mice in the HFD group that were not treated with Candida. This implies healthy mucosal barriers in non-CLP mice.