We also confirmed that circ003593 knockdown significantly activated PI3K-AKT signaling pathway and ERK1/2 pathway, both of which are the reperfusion injury salvage kinase (RISK) signaling pathway that is an important mechanism against myocardial ischemia/reperfusion injury (Hausenloy and Yellon, 2007). This evidence concerns the gene AKT1 and myocardial ischemia.