To further explore whether GDF11 plays an important role in adjusting cardiomyocytes pyroptosis in myocardial infarction, we evaluated the protein levels of NLRP3, ASC, cleaved-caspase-l (c-caspase-1), and GSDMD-N in ischemic heart and hypoxia cardiomyocytes with or without GDF11 treatment. Here, NLRP3 is linked to myocardial infarction.