In tongue cancer, mTORC2 downregulated miR-491-3p expression by inactivating FOXO1 (Zheng et al. 2015); while in osteosarcoma miR-491-3p functions as a tumor suppressor to attenuate the potential of growth and invasion by targeting TSPAN1 (Duan et al. 2017). Here, TSPAN1 is linked to osteosarcoma.