To validate our findings that CD9 expression (marker of the DAM cluster26) primarily increased in WT cells (not TREM2 KO) in the AD model brain (not WT) we performed flow cytometry for CD9 in all 4 sets of animals (MITRG + WT, MITRG + KO, 5x-MITRG + WT, 5x-MITRG + KO). This evidence concerns the gene TREM2 and Alzheimer disease.