We first performed assays in HCT116 cells to separate the cytoplasmic and nuclear RNA components and measured the expression ratio of LUCAT1 by qRT-PCR to confirm the subcellular localization of LUCAT1. We found that LUCAT1 was predominantly enriched in the nuclei of CRC cells (Fig. 3A). The gene discussed is LUCAT1; the disease is colorectal carcinoma.